Fine mapping of Quantitative Trait Loci (QTLs) associated with Striga resistant genes in sorghum | Crop Management (Crop Varieties)

The root hemiparasitic weed Striga hermonthica is a serious constraint to sorghum production in the ECA. Fine mapping of QTLs associated with Striga resistance in sorghum innovation was undertaken to improve the precision of Marker Assisted Selection (MAS) to facilitate breeding of sorghum for striga weed resistance. In this innovation, a total of 169 SSR markers associated with Striga resi Read more..

Description of the technology or innovation

The root hemiparasitic weed Striga hermonthica is a serious constraint to sorghum production in the ECA.  Fine  mapping  of  QTLs  associated  with  Striga  resistance  in  sorghum  innovation  was undertaken to improve the precision of Marker  Assisted Selection (MAS) to facilitate breeding of sorghum for striga weed resistance. In this innovation, a total of 169 SSR markers associated with Striga  resistance  QTLs  were  identified.  Of  these,  approximately  21  SSRs  and  175  DArT  markers were found to be closely associated with Striga resistance QTLs and were mapped. Fine mapping is the  fastest  and  currently  the  most  efficient  technique  for  generating  evidence  for  the  several
combinations of markers or association of markers that  support the existence of striga susceptibility genes in the region. Development and evaluation of the striga resistant lines was done in laboratories at ICRISAT and BeCA in Kenya. The innovation has been documented and is ready for up scaling among  sorghum  breeding  laboratories  of  the  ECA.  The  technology  was  validated  using  the  base recombinant inbred line (RIL) population and in both cases, the markers linked to the same genomic region confirmed the marker association.

Assessment/reflection on utilization, dissemination & scaling out or up approaches used

The ultimate beneficiaries of this technology are researchers, students and biotechnology companies. Main  method  was  through  publication  of  research  findings  in  journals,  which  can  reach  wider circulation globally among sorghum breeders and researchers.

Essential partners/stakeholders) to be involved in the further scaling out/up    
The key partners needed in the scaling up process are:

  • Sorghum breeders  
  • Researchers
  • Students
  • Biotechnology companies

 

Current situation and future scaling up

Current extent of reach of intended users of the fine mapping innovation in sorghum breeding.The innovation has not reached other sorghum breeders in the region.

Challenges encountered in respect to further dissemination, adoption and scaling up/out of fine mapping tool

  • This is basic research which calls for awareness among policy makers to facilitate sustained funding
  • Availability of good molecular laboratory facilities
  • Availability of large grounds for field testing

Recommendation for addressing challenges

  • Create awareness among policy makers and end users on fine mapping innovation
  • Forge collaboration linkages with laboratories in advanced countries

Lessons learnt  

  • Need for early awareness by policy makers of the use of this modern tool in the development of striga resistant sorghum varieties.
  • There is need for forge collaboration linkages with advanced laboratories for testing the fine mapping innovations.
  • Social, environmental, policy and market conditions necessary to catalyse fine mapping work
  • Programmes that increase policy makers awareness
  • Conducive institutional mechanisms for variety release

Economic Considerations

Basic  costs  (local  currency  and  equivalent  US  $)  associated  with  utilization  of  the  fine  mapping innovation  
Not done
Estimated returns (local currency and equivalent US$) such as cost benefit or gross margin figures where applicable
Not done

Gender considerations

Gender issues /concerns (if any) were considered in the fine mapping exercise
Was not part of the study
Gender issues/concerns  (if any) in the fine mapping exercise
Was not part of the study
Gender-related opportunities (if any), which enhanced or can enhance the genetic linkage mapping  
Was not part of the study

Case study or profiles of success stories

Success stories from beneficiaries attesting to the use of the genetic linkage mapping
None as yet
Methodologies for development genetic linkage map  
Are well documented and are being published

Contact details

Mohamed H Abdalla
Associate Professor, Agricultural Research Cooperation (ARC)
P.O Box 126
Khartoum, Sudan
Mob: +249-921934900
Email: abdalla_moh2002@yahoo.com; abdallamoh2002@gmail.com

Dr. Rasha Ali
ASARECA TIMPS-Revised Nov 2014  Page 51
Associate, Agricultural Research Co-operation (ARC)
P.O Box126
Khartoum, Sudan
Email: yarashaya@gmail.com

Dr Tom C. Hash
Sorghum Breeder
ICRISAT
Pantecheru,
India
Email: c.harsh@cgiar.org

Dr Santie de Villiers
Senior lecturer
Pwani University
Department of Chemistry and Biochemistry
PO Box 195-80108
Kilifi, Kenya
Tel: +254 729369758
E-mail: s.devilliers@pu.ac.ke
santiedevilliers@gmail.com

Dr. Dionysious (Dan) Kiambi
Executive Director
African Biodiversity Conservation and Innovations Centre (ABCIC)
P.O. Box 100882-00101, Nairobi, Kenya
Tel/Fax. 254-20-2330014
Mobile.254-731823394/704417920
Email:d.kiambi@abcic.org

 

Glossary

ANNEX 2
Glossary of terms
ARC:     Agricultural Research Cooperation of Sudan
ECA :    Eastern and Central Africa
MAS:     Marker Assisted Selection  
QTL:     Quantitative Trait Loci

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