BC2F1 generation with Striga resistance QTL | Crop Management (Crop Varieties)

This technology was developed by introgressing Striga resistanceQuantitative Trait Loci (QTL) from N13 line into an adapted sorghum varietyIS8193 using marker assisted backcrossing. Five Striga resistance QTLs weretargeted. The introgression was performed using Simple Sequence Repeats (SSRs)molecular markers. The BC2F1 generation carrying Striga resistance QTLs wasexpected to undergo further genotyping, backcrossing and selfing in order toproduce farmer Read more..

Description of the technology or innovation

This technology was developed by introgressing Striga resistanceQuantitative Trait Loci (QTL) from N13 line into an adapted sorghum varietyIS8193 using marker assisted backcrossing. Five Striga resistance QTLs weretargeted. The introgression was performed using Simple Sequence Repeats (SSRs)molecular markers. The BC2F1 generation carrying Striga resistance QTLs wasexpected to undergo further genotyping, backcrossing and selfing in order toproduce farmer preferred sorghum lines resistant to Striga for adoption inRwanda and other countries in the ECA region. The technology was developed atthe University of Nairobi and at ILRI BecA laboratory.


Assessment/reflection on utilization, dissemination & scaling out or up approaches used

The ultimate beneficiaries of the technology are breeders who willuse it to carry out further backcrosses to adequately recover IS8193 backgroundand farmers who are expected to use released materials. The technology wasvalidated by genotyping to confirm introgression of the QTL in a heterozygousstate. This needed further validation through genotyping to confirm fixed QTLas well as phenotyping of the trait.


Current situation and future scaling up

The technology will be disseminated to farmers through farmergroups. Information about the technology will be disseminated to farmersthrough various platforms and information networks. Farmers will be trainedthrough TOTs on use of the technology to support future scaling. 


Economic Considerations

The technology will be instrumental in preventing losses caused byStriga, and increasing sorghum yields, thereby contributing to improved foodsecurity and household incomes of adopting farmers.


Gender considerations

The technology is considered gendersensitive. However, the women are more likely to benefit than men since they contributemost of the family labor that goes into crop production activities especiallyweeding.

Contact details

Theogene Niyibigira;

Scientist, Rwanda Agricultural Board (RAB);

P. O. Box BP 5016;

47 Rue Kamuzinzi;

Kigali, Rwanda.

Tel: 250-578768 /250-0830 4197

Fax: 250-578768

 

Eliud K. Ngugi;

Scientist, University of Nairobi (UON);

P.O. Box 29053;

Nairobi, Kenya.

Email: Kahiu.ngugi@yahoo.com

 

Eunice Mutitu;

Professor, University of Nairobi (UON);

P. O. Box 29053;

Nairobi, Kenya.

 

Santiede Villiers;

Scientist; International Crops Research Institute for theSemi-Arid Tropics (ICRISAT);

P. O. Box 30709-00100;

Nairobi, Kenya.

Email: s.devilliers@cgiar.org

 

Dan Kiambi;

Scientist, African Biodiversity Conservation & InnovationCentre (ABCIC);

P.O. Box 100882-00101;

Nairobi, Kenya.

Tel: +254-20-2330014

Mob: +254-722926086

Email: d.kiambi@abcic.org

Additional information


Figure 1: LR: Maize in a greenhouse and research being conducted in the laboratory


Glossary

Haussmann, B.I.G., Hess, D.E., Koyama, M.L., Grivet L., Rattude,H.F.W. and Geiger, H.H., 2000a. Breeding for Striga resistance in cereals.MargrafVerlag, Weikersheim, Germany.

 

Haussmann, B.I.G., Hess, D.E., Geiger, H.H. and Welz, H.G., 2000b. Improved methodologies for breeding Striga-resistant sorghums. (ReviewArticle). Field Crops Research. 66, 195-201.

 

UNDP, 2010. Millennium Development Goals Progress Report. RwandaCountry Report. 2010.


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